Identification and handling of OCCC during egg recovery.
Embryology
IVF Insemination
Correct identification of sperm and OCCC and their mixture
Embryology
ICSI
The correct identification of sperm and OCCC, the denuding of the oocyte, the set up of the injection equipment and the injection of the oocyte by a single spermatozoon.
Embryology
Fertilisation Check
Correct Identification, removal of granulosa material, correct identification of pronuclei and transfer of embryos to new culture media.
Embryology
Blastocyst culture
Correct identification of embryos, preparation of culture dishes,movement of embryos, correct grading of embryos for transfer, cryostorage or discard
Embryology
Assisted Hatching
Either laser or Acid Digestion, cleavage or blastocyst stage, fresh or frozen embryos. Includes complete or partial hatching.
Embryology
Freezing of Cleavage Embryos
Correct patient identification, embryo grading of pronuclear or cleavage stage embryos, dehydration of embryos,loading into straws, operation of freezer and movement to storage tanks. Documentation.
CryoBiology
Blastocyst Freezing
Correct patient identification and blastocyst grading,dehydration of embryos and loading into cryotools(straws), operation of freezer and movement to storage tanks. Completion of documentation.
CryoBiology
Thawing of cleavage stage embryos
Correct patient identification, location of pronuclear or cleavage stage embryos in storage tanks, re-hydration of embryos, transfer to culture media. Documentation
CryoBiology
Blastocyst Thawing
Correct patient identification, location of blastocysts in storage tanks, rehydration of embryos, transfer to culture media. Documentation
CryoBiology
Vitrification of cleavage stage embryos
Correct patient identification, embryo grading of pronuclear or cleavage stage embryos, their vitrification and loading onto cryotools, and their movement to storage tanks. Documentation.
CryoBiology
Blastocyst vitrification.
Correct patient identification, embryo grading of blastocysts, their vitrification and loading onto cryotools, and their movement to storage tanks. Documentation.
CryoBiology
Warming of Cleavage Stage Embryos
Correct identification and warming of pronuclear or cleavage stage embryos, assessment of embryos, movement to culture media. Documentation
CryoBiology
Blastocyst Warming
Correct identification and warming of blastocysts, assessment of embryos, movement to culture media. Documentation
CryoBiology
Oocyte Freezing
Correct identification and assessment of mature oocytes, dehydration, loading into straws,operation of freezer and movement to storage tanks. Documentation
CryoBiology
Oocyte Thawing
Correct patient identification, location of oocytes in storage tanks, their re-hydration, transfer to culture media. Documentation
CryoBiology
Oocyte Vitrification
Correct patient identification, grading of mature oocytes, their vitrification and loading onto cryotools, and their movement to storage tanks. Documentation.
CryoBiology
Oocyte Warming
Correct identification and warming of oocytes, their post-warm assessment, movement to culture media. Documentation
CryoBiology
Blastocyst Biopsy
Zona breaching and removal of one or more trophectodermal cells and preparation for FISH or PCR.
PGD
Cleavage Stage Biopsy
Removal of one or more blastomeres of day 3 cleavage stage embryos and preparation for FISH or PCR.
PGD
Polar Body Biopsy
Removal of one or more polar bodies and preparation for FISH or PCR
