Comment:Main focus on this weeks IVFdaily posting centres around witnessing of dishes in IVF. This coincides with the release of a new education module on Sample Witnessing as part of the 2014 IVFCPD ongoing education program.
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Assisted oocyte activation following ICSI fertilization failure
This review tackles the mechanism of human oocyte activation and the relatively rare phenomenon of fertilization failure after ICSI. Next, we describe the current diagnostic approaches and focus on the application, efficiency and safety of AOA in human assisted reproduction.
Oocyte banking for anticipated gamete exhaustion (AGE) is a preventive intervention, neither social nor nonmedical
The scope of female fertility preservation through cryopreservation of oocytes or ovarian cortex has widened from mainly oncological indications to a variety of fertility-threatening conditions. So far, no specific universally accepted denomination name has been given to cryopreservation of oocytes or ovarian cortex for the prevention of age-related fertility decline. We argue that the commonly used phrases ‘social’ and ‘nonmedical freezing’ to d
Rapid warming increases survival of slow-frozen sibling oocytes: a step towards a single warming procedure irrespective of the freezing protocol?
Nowadays, human oocytes/embryos are cryopreserved via slow freezing or vitrification. The aim of this study was to evaluate a rapid warming protocol for slow-frozen human oocytes based on the standard warming procedure for vitrification - - Survival rate with rapid warming was higher (92/102, 90.2%) than with rapid thawing (85/114, 74.6%: P = 0.005), and after 3 d of culture the rapidly warmed parthenotes had more blastomeres compared with those
Decisions for the IVF laboratory: comparative analysis of embryo culture incubators
This review highlights incubator function and reviews key environmental variables controlled and the technology utilized in various units. Additionally, existing comparative studies focused on incubator recovery and clinical outcomes are critically analysed. Finally, strategies employed for incubator management, as well as future potential incubator improvements, are discussed. While existing reports indicate that smaller benchtop/topload incubat
Protocols for tracking and witnessing samples and patients in assisted reproductive technology
After some adverse events involving sample misidentification occurred in some countries, double-checking every step of the IVF clinical and laboratory procedure has become mandatory. However, double-checking protocols can also prove difficult to implement and a new generation of errors may occur. Other approaches, including electronic strategies for tracking, and even microlabeling embryos, are currently being evaluated.
Morphokinetic parameters of ICSI tripronucleated embryos observed using time lapse
Time-lapse analysis of tripronucleated zygotes obtained in ICSI cycles showed that 75.4% cleaved into embryos. These embryos subsequently demonstrated slower developmental kinetics than normally fertilized embryos.
STANDARD: WITNESSING THE IDENTIFICATION OF SAMPLES AND PATIENTS/DONORS
Centres shall have witnessing protocols in place to double check the
identification of samples and the patients or donors to whom they relate at all critical points of the clinical and laboratory process. These checks shall be completed and recorded at the time the clinical or laboratory process/procedure takes place.
A novel embryo identification system by direct tagging of mouse embryos using silicon-based barcodes
Barcodes present in the perivitelline space, independently of their type and number, did not affect embryo development rates. The majority of embryos (>90%) retained at least one of the microinjected barcodes in their perivitelline space up to the blastocyst stage. Increasing the number of barcodes per embryo resulted in a significant increase in embryo identification rates, but a significant decrease in the barcode release rates after embryo hat
Direct embryo tagging and identification system by attachment of biofunctionalized polysilicon barcodes to the zona pellucida of mouse embryos
Neither the in vitro or in vivo development of tagged embryos was adversely affected. The tagging system also proved effective during an embryo cryopreservation process. / The direct embryo tagging system developed here, once tested in human embryos, could provide fertility clinics with a novel tool to reduce the risk of mix-ups in human assisted reproduction technologies.
Barcode tagging of human oocytes and embryos to prevent mix-ups in assisted reproduction technologies
The direct embryo tagging system developed here provides fertility clinics with a novel tool to reduce the risk of mix-ups in human ARTs. The system can also be useful in research studies that require the individual identification of oocytes or embryos and their individual tracking.
The risk management process should focus on minimizing losses, including staff injury, premature warming of cells and tissues, mistaken identity, and transmission of infection. Early warning and monitoring systems should be in place for quality assurance and to prevent incidents involving cryovessels turning critical. Centres must ensure that every reasonable practical measure that can be put in place is done so, and that resourcing of the servic
Quality indicators must be developed to monitor contributions to patient care and continual improvement. We discuss the implementation of ISO 15189 with a specific emphasis on the PGD laboratory, highlight elements of particular importance or difficulty and provide suggestions of effective and efficient ways to obtain accreditation. The focus is on the European environment although the principles are globally applicable.
Next Evolution in Fertility Requires Investment in Air
When one of the first fertility clinics in the Bay Area moves to its new home next month, one of the newest – and arguably most innovative – features is one that can't be seen. As part of Reproductive Science Center of the Bay Area's (RSCBA) multimillion-dollar build, a considerable investment in technology and facility design has been dedicated to providing the best possible air quality to support embryo growth and development.
IVF treatment methods are currently in evolution mode, though it would appear that governments of major developed nations like the US and UK, just aren't sure where to draw the line when it comes to development and research into IVF treatments, leaving the door wide open to IVF research abroad. Within Europe itself, Spain and Bulgaria lead the way into IVF research, but if we take a look at what is currently happening in Israel and Nigeria, the p
Patients out of pocket after sudden closure of fertility clinic
Patients at a fertility clinic have been left without access to their medical files, and some out of pocket by several thousand euro, after it shut its doors suddenly without notice.Emergency measures kicked into place, with sperm and embryos safely transferred to another facility after the Kilkenny Clinic Ltd – which offered IVF treatment and freezing services – closed on January 13. The clinic states it has shut due to the serious ill health of
Dogan and Victoria Baylam, of Kirkstone Avenue, suffered the pain of two unsuccessful cycles of IVF before saving up for three years to pay £7,000 for another attempt. The new technique called Eeva (Early Embryo Viability Assessment) at Liverpool Women’s Hospital meant it was third time lucky for the couple who finally welcomed Leyla Eva to the world on January 13 weighing 6lb 3oz.
Fertility MDs warn some doctors performing inseminations, fertility treatments outside scope of practice
The nation’s fertility specialists are calling for minimum qualifications for doctors offering assisted baby-making procedures, saying that doctors who don’t have the proper training to perform the procedures are treating some patients. [Canada]
Scientific Publications
IVF and Early Embryo Viability Assessment are driven by core research and scientific discovery. The learning and advancements continue to be covered in peer-reviewed publication. The most relevant publications are provided below.
Mobeus Equity Partners invest £3.5 in Bourn Hall IVF clinic expansion
Bourn Hall, the world’s first IVF clinic, has teamed up with Mobeus Equity Partners who have invested £3.5m, with more to come to fund expansion.
The plans include acquisition and partnership with existing IVF providers and the development of several new fertility centres and satellite clinics.
Leading UK newspapers The Times and Daily Mail have this week both reported on a new IVF treatment breakthrough made by scientists, who have managed to discover the chemical signal which predicts whether an embryo will be accepted by the womb or not. The chemical given off by promising embryos called Trypsin has been proved to be responsible for preparing the womb for implantation, which will help to show Doctors if the womb will reject an embryo
Soon, 3D technique to sort fast sperms from slow ones
Here comes a tracking system that makes 3D movies of living sperm to detect potential infertility-causing anomalies – such as the ‘bent tail’ that prevents the cells from swimming straight, reports IANS. - In their new technique, the team of researchers from Italy and Belgium combined microscopy and holography – the creation of 3D images – to visualise live sperm in not only two dimensions but according to their depth as well.
OvaScience's Augment fertility treatment receives two US patents
OvaScience, a life sciences company focused on the discovery, development and commercialization of female fertility treatments, has received two United States patents covering its Augment fertility treatment. Augment is based on the company’s egg prec`ursor cell (EggPC) technology and is being developed to improve egg quality and the success of in vitro fertilization (IVF). Augment will be available in international IVF clinics this year
Continuous Professional Development Modules-Sample Identification-Dr Steve Fleming
This review will consider the rationale for witnessing, the mode of operation of the main witnessing systems currently available, and the relative strengths and weaknesses of witnessing systems depending upon the manner in which they are applied.
In-line filter for cylinder and house gasses
O2, N2, CO2, and tri-gas. Aire~LifeLine provides in-line air filtration with top of the line protection, delivering a better and more consistent yield of cylinder and house gasses for a wide range of med
Vit Kit - Warm NX is an adaptable, cost-effective system for use in the thawing of oocytes, pronuclear zygotes, cleavage stage embryos, and blastocyst stage embryos. Unlike many vitrification kits that feature a mono-buffered system and M199 base med
Vit Kit - Freeze NX is an adaptable, cost-effective system for use in the vitrification of oocytes, pronuclear zygotes, cleavage stage embryos, and blastocyst stage embryos. Vit Kit - Freeze NX is the latest advancement in vitrification media aimed t
S-Cryolock is the slimmer version of the original. It is a versatile, simple and efficient vitrification device that is intended for the holding, cryopreservation and storage of oocytes or embryos in liquid nitrogen.
Source
Irvine Scientific
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