Comment:Several links to article discussing oxygen and culture media. Reading these strongly agues that all IVF should be at 5%O2. Plus another link to pyruvate/lactate ratios impact on PN stage embryos. Fascinating. Plus 2 talks from ESHRE RI Stand of interest. Finally, Fertaid is developing an elearning module. Try the July 2014 current topics quiz.
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Sperm involved in recurrent partial hydatidiform moles cannot induce the normal pattern of calcium oscillations
Sperm that previously provoked recurrent partial hydatidiform mole pregnancies due to dispermic fertilization is not able to activate human oocytes or trigger the normal pattern of Ca2+ oscillations in mouse and human oocytes in vitro.
Clinical rationale for cryopreservation of entire embryo cohorts in lieu of fresh transfer
Recent dramatic increases in success rates with frozen–thawed embryo transfer (FET) are encouraging, as are numerous findings of several reduced risks with FET when compared with fresh transfer. These reduced risks include low birth weight and prematurity, among others. However, FET is also associated with increased risks of macrosomia and large for gestational age. There have been reports of greater implantation and pregnancy rates with FET than
Patient satisfaction concerning assisted reproductive technology treatments in moderate to severe endometriosis.
Only three patients would refrain from this preceding therapy in a next treatment attempt. We concluded that patient satisfaction scores were comparable between the three different ART treatments. Since patient satisfaction was in particular dependent on treatment outcomes, it is recommended to compare those three ART treatments in a randomized controlled trial investigating the efficacy, safety and cost-effectiveness.
The use of portable CO2 incubator for cross-border shipping of embryos in an international egg donation program.
The clinical pregnancy rate (fetal heart beat) was 48.1 and 43.1% per embryo transfer cycle, respectively (NS) and the delivery rate was 44.1 and 35.9% per embryo transfer cycle, respectively (p := :0.01). Long distance transportation of human pre-implantation embryos using portable CO2 incubator is safe and do not jeopardize their developmental potential.
Effect of pretreatment with transdermal testosterone on poor ovarian responders undergoing IVF/ICSI: A meta-analysis
The meta-analysis revealed that females who received transdermal testosterone treatment prior to their IVF/ICSI cycle had a two‑:fold increase in live birth rate [risk ratio (RR)=2.01, 95% confidence interval (CI) 1.03‑:3.91], clinical pregnancy rate (RR=2.09, 95% CI 1.14‑:3.81) and a significantly more oocyte retrieved [mean difference (MD)=1.36, 95% CI 0.82-1.90]. The current findings provide evidence that pretreatment with tran
Oxygen Regulates Amino Acid Turnover and Carbohydrate Uptake During the Preimplantation Period of Mouse Embryo Development (Archive, 2012)
These metabolic changes correlated with developmental compromise because embryos grown in atmospheric oxygen at all stages showed significantly lower blastocyst formation and proliferation. These findings confirm the need to consider both embryo development and metabolism in establishing optimal human embryo growth conditions and prognostic markers of viability, and further highlight the impact of oxygen on such vital parameters.
Oxygen Affects the Ability of Mouse Blastocysts to Regulate Ammonium
Together, 5% oxygen and ammonium promoted glutamine production, whereas in the presence of 20% oxygen and ammonium, glutamine was consumed. Data reveal that both oxygen and ammonium affect amino acid utilization by the developing embryo, however, 20% oxygen appears to have the greater impact. Mouse blastocysts can alleviate ammonium stress by its transamination to both glutamine and alanine, but only under physiological conditions.
The transition from 20% to 5% oxygen was robust. The 20% oxygen concentration clearly induces global changes in mRNA expression [25], and incorrect oxygen levels and other stresses induce loss of potency at all stages of embryo culture and in stem cells derived from the blastocyst [12, 19]. The next challenges will be determining a dose response to oxygen concentrations because this study focused on historical changes in oxygen concentrations but
Adult Body Weight Is Programmed by a Redox-Regulated and Energy-Dependent Process during the Pronuclear Stage in Mouse (Archive:from 2011)
We report that experimentally manipulating the redox potential of fertilized eggs during the pronuclear (PN) stage affects post-natal body weight. - Exogenous pyruvate induces NAD(P)H oxidation and stimulates mitochondrial activity with resulting offspring that are persistently and significantly smaller than controls
Composition of commercial media used for human embryo culture
Culture media composition varies widely, with differences in pyruvate, lactate, and amino acids especially notable. Blastocyst development was culture media dependent and showed an interaction with oxygen concentration and presence of protein.
Chromosome analysis in embryos from young patients with previous parity
The level of FISH sensitivity was calculated in the group of term pregnancies where the number of euploid embryos was expected to exceed or match with the number of babies born. The resulting false-negative rate was 4.0% per patient and 1.9% per embryo. These findings confirmed the limited prediction power of embryo morphology on implantation but also the relevance of chromosomal abnormalities in causing embryo demise.
Is assessment of anti-mullerian hormone and/or antral follicle count useful in the prediction of ovarian response in expected normal responders treate
Logistic regression analysis showed that only when AMH was added to the base model, including age and FSH, its predictive capacity for high ovarian response was statistically significant (F-test: p := :0.001: c-statistic: 0.80, 95% CI: 0.70–0.88), but this was not the case for poor ovarian response. In conclusion, the addition of AMH, but not of AFC, to a model including female age and basal FSH, is useful in the prediction of ovarian r
Letrozole versus Clomiphene for Infertility in the Polycystic Ovary Syndrome
As compared with clomiphene, letrozole was associated with higher live-birth and ovulation rates among infertile women with the polycystic ovary syndrome.
Letrozole may help women with polycystic ovary syndrome become pregnant
The drug letrozole results in higher birth rates in women with polycystic ovary syndrome (PCOS) than the current preferred infertility treatment drug, according to a nationwide American study. PCOS affects 5 to 10 percent of reproductive-age women and is the most common cause of female infertility. Women affected have excessive levels of the hormone androgen, have infrequent periods, develop small cysts on the ovaries and have trouble conceiving.
Thousands of women trying for a baby through IVF are undergoing unnecessary and expensive procedures to examine the womb, warn doctors. Research shows that having a hysteroscopy to check the womb before fertility treatment makes almost no difference to success rates.
The former adviser to Tony Abbott who proposed the controversial GP co-payments has called for limits to the funding of reproductive medicine, potentially putting the profitability of newly listed stocks Virtus Health and Monash IVF at risk. Terry Barnes, who is a consultant and advised Mr Abbott when he was health minister in the Howard government, said the $550 million fertility medicine sector, which is dominated by the two highly profitable p
Adult Body Weight Is Programmed by a Redox-Regulated and Energy-Dependent Process during the Pronuclear Stage in Mouse
Summary
This study has shown that manipulation of the pyruvate/lactacte ratios on pronuclear mouse embryos influences the birthweight of offspring
Comment
This study may well pass many embryologists by because many would not understand the role of pyruvate and lactate or their ratios and because no-one knows the ration in the culture media they are using. However a recent report by Morbeck (2104, Fertil & Steril) has identified large variations in the P:L ratio between media manufacturers. Suddenly, this study raises some sever questions about the choice of culture media and the health of the children being generated in IVF. This study suggests that an incorrect ratio at the time of fertilisation and pronuclear development may predispose the offspring to lower or higher birth weight. Makes one wonder a bit more about culture media, knowledge and public disclosure and reinforces a view that every aspect of IVF deserves repeated review. Who would have thought the redox potential of the just fertilised oocyte may influence the birthweight of children 9 months+ later
IVF Training - Questions relating to mailout links -
Open Challenge and complete Questions
Question
Type
Options
Which markers predict ovarian response in normal responders
2
5
Mild to moderate Alcohol (>3 drinks/week) consumption may be linked to a decreased live birth rate
1
2
The use of a portable CO2 incubator to transport oocytes (Donor) or embryos does not appear to be detrimental
1
2
Letrozole is superior to Clomiphene in ovulation rates and pregnancy for women with Polycystic Ovary Syndrome
1
2
Letrozole is superior to Clomiphene in ovulation rates and pregnancy for women with Polycystic Ovary Syndrome
1
2
All culture media are basically the same
1
2
The pyruvate/lactate ratio has significant impact on the offspring birth weight
1
2
Culture O2 concentrations can influence amino acid and carbohydrate metabolism (in the mouse)
1
2
All culture media behaves the same at either 5% or 20% oxygen
1
2
In the mouse, oxygen concentration causes differences in how an embryo metabolises amino acids and carbohydrates
1
2
Mouse blastocysts can alleviate ammonium stress by its transamination to both glutamine and alanine, but only under physiological conditions of 5% oxygen.
1
2
Which oxygen concentrations are used in IVF
2
2
Which is thought to represent the tubal oxygen concentration
1
2
In the mouse, oxygen concentration causes differences in how an embryo metabolises ammonia
1
2
The live birth rate in human has been shown to be the same at 5% and 20% oxygen
1
2
Between 25 -50% of clinics use 5% CO2 in air as a part or total incubator environment.
1
2
There are some suggestions 20% oxygen (ambient) may be a source of reactive oxygen species.
1
2
If you culture at 5% oxygen, then every time an incubator door is opened the following occurs:
1
6
If You Culture At 20% Oxygen (ambient), Then Every Time An Incubator Door Is Opened The Following Occurs:
1
6
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S-Cryolock is the slimmer version of the original. It is a versatile, simple and efficient vitrification device that is intended for the holding, cryopreservation and storage of oocytes or embryos in liquid nitrogen.
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