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Exposure of embryos to oxygen at low concentration in a cleavage stage transfer program: reproductive outcomes in a time-series analysis.
For ICSI procedures, no significant difference in ART outcomes was found between the two culture conditions. For conventional IVF cycles, both fertilization rate (59 +/- 36 vs. 71 +/- 32, respectively) and proportion of embryos obtained (38% vs. 50%, respectively) were significantly improved under low oxygen condition.
Markers of stem cells in human ovarian granulosa cells: is there a clinical significance in ART?
Expression of OCT-4 mRNA, which is a typical stem cell marker and absence of expression of DAZL mRNA, which is a typical germ cell marker, suggest that a subpopulation of luteinized granulosa cells in healthy ovarian follicles (47.6%) consists of stem cells, which are not originated from primordial germ cells. Absence of Oct-4 gene expression in more than half of the cases means probably the end of the productive journey of these cells, towards t
Ovarian hyperstimulation syndrome and prophylactic human embryo cryopreservation: analysis of reproductive outcome following thawed ET
For these patients, immediate elective embryo cryopreservation and delay of ET by as little as 30 d allowed for satisfactory conclusion of the IVF sequence, yielding a livebirth-delivery rate (per ET) >40%.
Conclusion: Vitamin-E or L-arginine treatment improved luteal function by decreasing CL blood flow impedance. CL blood flow is a critical factor for luteal function.
Clomiphene citrate in combination with gonadotropins for controlled ovarian stimulation in women undergoing in vitro fertilization
There was no evidence to indicate that clomiphene with gonadotropins (with or without GnRH antagonist) differed significantly from gonadotropins in GnRH agonist protocols for women undergoing IVF treatment, in terms of live births or pregnancy rates. Meanwhile, use of clomiphene led to a reduction in the incidence of OHSS. However, these results were based on data from a small number of underpowered randomised trials with few participants. Hence
In vitro fertilisation for unexplained subfertility
IVF may be more effective than IUI+SO. Due to paucity of data from RCTs the effectiveness of IVF for unexplained infertility relative to expectant management, clomiphene citrate and IUI alone remains unproven. Adverse events and the costs associated with these interventions have not been adequately assessed.
Luteal phase support for assisted reproduction cycles
This review showed a significant effect in favour of progesterone for luteal phase support, favouring synthetic progesterone over micronized progesterone. Overall, the addition of other substances such as estrogen or hCG did not seem to improve outcomes. We also found no evidence favouring a specific route or duration of administration of progesterone. We found that hCG, or hCG plus progesterone, was associated with a higher risk of OHSS. The use
Low oxygen concentrations for embryo culture in assisted reproductive technologies
The results of this systematic review and meta-analysis suggest that culturing embryos under conditions with low oxygen concentrations improves the success rates of IVF and ICSI, resulting in the birth of more healthy newborns.
Cleavage stage versus blastocyst stage embryo transfer in assisted reproductive technology
This review provides evidence that there is a small significant difference in live birth rates in favour of blastocyst transfer (Day 5 to 6) compared to cleavage stage transfer (Day 2 to 3). However, cumulative clinical pregnancy rates from cleavage stage (derived from fresh and thaw cycles) resulted in higher clinical pregnancy rates than from blastocyst cycles. The most likely explanation for this is the higher rates of frozen embryos and lower
Elective frozen replacement cycles for all: ready for prime time?
In this article we explore the biological plausibility of this concept, appraise the evidence underpinning it and consider the implications of adopting such a strategy in routine clinical practice. The outcomes of existing randomized trials appear to favour a strategy of frozen embryo transfer, but larger trials are needed before a major change in clinical practice can be considered.
Embryos whose polar bodies contain isolated reciprocal chromosome aneuploidy are almost always euploid
This study is consistent with increasing evidence that PSSC is the primary cause of meiosis I errors in embryos from women of advanced reproductive age. Clinicians should be cautious in interpreting results from polar body aneuploidy screening, especially when only the first polar body is tested.
Neonatal outcome and congenital malformations in children born after ICSI with testicular or epididymal sperm: a controlled national cohort study
Does neonatal outcome including congenital malformations in children born after ICSI with epididymal and testicular sperm [testicular sperm extraction (TESE)/percutaneous epididymal sperm aspiration (PESA)/testicular sperm aspiration (TESA) (TPT)] differ from neonatal outcome in children born after ICSI with ejaculated sperm, IVF and natural conception (NC)?
Coitus to expedite the onset of labour: a randomised trial
Objective: To evaluate the effect of suggesting coitus as a safe and effective means to expedite labour on pregnancy duration and requirement for labour induction.Conclusions: Labour onset and labour induction did not differ in the advise-coitus arm.
The dynamics of PKC-induced phosphorylation triggered by Ca2+ oscillations in mouse eggs
These data show that PKC-induced phosphorylation outlasts each Ca2+ increase in mouse eggs but that signal integration only occurs at a non-physiological, high Ca2+ oscillation frequency. The results also suggest that Ca2+-induced DAG formation on intracellular membranes may stimulate PKC activity oscillations at fertilization.
Unhealthy Changes in Gut Microbes Benefit Pregnant Women
In the context of pregnancy, these metabolic changes in the mother are healthy, because they promote energy storage in fat tissue and help support the fetus. Outside of pregnancy, however, these changes can lead to the development of type 2 diabetes and other health problems.
High Vitamin D Levels in Pregnancy May Protect Mother More Than Baby Against Multiple Sclerosis
Pregnant women who have higher levels of vitamin D in their blood may have a lower risk of developing multiple sclerosis (MS) than women with lower levels, while their babies may not see the same protective effect, according to a study published in the November 20, 2012, online issue of Neurology®, the medical journal of the American Academy of Neurology.
A University of Newcastle biology professor has been named the New South Wales Scientist of the Year. Laureate Professor John Aitken has taken out the top award at the 2012 Science and Engineering awards in Sydney overnight.
There is a widespread belief that sex during the later stages of pregnancy can jumpstart labour, but that doesn't appear to be so - at least according to a study from Malaysia.
Sperm DNA Fragmentation SURVEY [This link may be from a sponsor]
Assessment of Sperm DNA Fragmentation by TUNEL, SCSA, halosperm or other appear very popular but is it? Complete the survey to find out just how many clinics are performing this assay. [Tell your fellow scientists to complete this survey!!]
Expanded Halosperm EQA program. [This link may be from a sponsor]
An EQA/Competency schemes for the assessment of sperm DNA fragmentation by the Halospem method. This has been expanded to 12 data sets for 2013 with images form several users. Participants are shown 20+ sets of images from one sample and asked to estimate the number Normal and abnormal sperm ( according to the methodology) and calculate a % DFI
In-line filter for cylinder and house gasses
O2, N2, CO2, and tri-gas. Aire~LifeLine provides in-line air filtration with top of the line protection, delivering a better and more consistent yield of cylinder and house gasses for a wide range of med
Vit Kit - Warm NX is an adaptable, cost-effective system for use in the thawing of oocytes, pronuclear zygotes, cleavage stage embryos, and blastocyst stage embryos. Unlike many vitrification kits that feature a mono-buffered system and M199 base med
Vit Kit - Freeze NX is an adaptable, cost-effective system for use in the vitrification of oocytes, pronuclear zygotes, cleavage stage embryos, and blastocyst stage embryos. Vit Kit - Freeze NX is the latest advancement in vitrification media aimed t
S-Cryolock is the slimmer version of the original. It is a versatile, simple and efficient vitrification device that is intended for the holding, cryopreservation and storage of oocytes or embryos in liquid nitrogen.
Source
Irvine Scientific
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