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Mail Name:IVFDaily 2018.26 EQA-Time Lapse Assessment
Distribution: 7821-7825 Posted 31/5/2018 Embryo Ranking Challenge-Invitation
Comment: Survey-2014 Laboratory Census
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IVFD Sperm selection by thermotaxis improves ICSI outcome in mice
Collectively, our results indicate that a high quality sperm subpopulation is selected in vitro by thermotaxis and that this subpopulation is also selected in vivo within the fallopian tube possibly by thermotaxis.
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- View Link - Keywords: thermotaxis ICSI sperm selection
Publications / [Posted:31/5/2018] / [Source:www.nature.com] Viewed: 28
IVFD Secretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side
Communication between embryo and maternal endometrium occurs during a specific time frame in which implantation is possible. Here we demonstrate for the first time that conditioned media from non-manipulated human embryos cultured in vitro for 3 days or up to the blastocyst stage contain extracellular vesicles (EVs) with a diameter of 50 to 200 :nm and bearing the traditional microvesicle and exosome marker proteins CD63, CD9 and ALIX.
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- View Link - Keywords: Secretome extracellular vesicles endometrium
Publications / [Posted:31/5/2018] / [Source:www.nature.com] Viewed: 24
IVFD Women sometimes feel regret after electing to freeze their eggs - Experts urge caution as technology to defer childbearing spreads across America
Most women feel empowered by elective procedures that enable them to bank eggs in case they can't conceive naturally later in life, researchers have found. But one in six become regretful, for reasons that researchers do not yet fully understand.
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- View Link - Keywords: Egg Freezing
News Articles / [Posted:31/5/2018] / [Source:www.sciencedaily.com] Viewed: 29

Comments Linked to this Mailout
Title
Can Sperm selection by thermotaxis improve ICSI outcome?
Summary
Sperm selected by a warmer temperature gradient appear to have less DNA damage, better qualities and possibly better embryo quality.
Comment
Currently, the intracytoplasmic sperm injection (ICSI) is the preferred Assisted Reproductive Technology (ART) for the treatment of infertility in humans. Furthermore, the interest for its development in farm animals is rising for improving the management of reproductive processes. However, ICSI still inefficient and reports low percentages of pregnancies to term respect to the number of produced embryos. In addition, a concomitant risk of deleterious effects during the life of ICSI derived offspring has been pointed out. It has been suggested that one of the reasons for these issues is the lack of an effective technique for selecting the spermatozoa. This implies a high probability of injecting spermatozoa with damaged DNA explaining both deleterious and lethal effects on embryos and born descendants. Here we present a novel approach in vitro for the selection of the sperm fraction able to migrate within a temperature gradient by thermotaxis. We show in mouse and human that the DNA ported by this fraction is of much higher integrity than before separation. Moreover, using this selected spermatozoa we achieved a substantial improvement of the ICSI outcome in mouse. Finally we found that this sperm subpopulation show the same distribution of the thermosensor rhodopsin than the spermatozoa selected in vivo within the mouse oviduct suggesting that thermotaxis operates as both guidance and selection mechanism of spermatozoa in vivo. The results discussed here could be of high impact at different social, economic and scientific levels. The use of thermotaxis for selecting spermatozoa opens new possibilities for improving ARTs, increasing the success of the fertility treatments in human and veterinary clinics, and reduce the risk of long term effect associated with ICSI. Furthermore, separation of the thermotactic responsive spermatozoa allows analyzing in deep these capacitated cells which will provide novel and valuable knowledge on basic sperm biology.


IVFLabOnline - Recent Enteries to IVFLabOnlines product listings. see IVFLabonline
Product
iVitri-EZ Vitrification Straw View
Description
iVitri® -Straw is US patent and registered product (USPTO), specifically designed for vitrification, which can be used for freezing oocyte and embryos. The device tip is made of special material and in a curved shape for keeping gametes and embryo i  
Source
Sunlight Medical
   
Product
SperMagic® Medium [Research only] View
Description
Improving sperm function like magic. SperMagic® can enhance Ca2+ influx via membrane Ca2+ channel, consequently increase dynamic movement of micro-tubules through signal transduction system, and finally promotes sperm motility. SperMagic® could bin  
Source
Sunlight Medical
   
Product
ICSI Injection Pipettes View
Description
 
Source
Sunlight Medical
   
Product
Cell-Tek Microscope Chambers View
Description
Optimising embryo working environment using the latest technology - Cell-Tek chambers are an essential part of good laboratory practise when visually examining, manipulating, transferring or assessing oocytes and embryos for in-vitro fertilisation  
Source
Tek-Event Pty Ltd
   
Product
Arctic™ Sperm Cryopreservation Medium View
Description
Arctic Sperm Cryopreservation Medium is a TYB-free, dual-buffered medium designed to be effective at a low 3:1 ratio of semen to medium.  
Source
Irvine Scientific
   

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