Comment:A few links to pH issues, another news article on IVF in Japan and an IVF.net comment on CRISPR technology.
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Validation of the measurement of a critical parameter in human embryo culture: pH [In french - may need Google translate!]
Collaboration between the biochemistry and reproductive biology departments made it possible to undertake an automated measurement of the pH of the culture medium on a blood gas analyzer. This method has been validated and evaluated for its performance. It is applicable in all laboratories whatever the medium and the conditions of culture. It allows a strict monitoring of this parameter for the optimization of the culture conditions necessary to
CRISPR editing human embryos - did it work or not?
Controversy surrounding last year's report of a disease gene being edited out of human embryos continues with a fresh round of evidence. A group led by Dr Shoukhrat Mitalipov of the Oregon Health and Science University in Portland published a study in Nature last year describing how they used CRISPR/Cas9 genome editing to repair a genetic variant in human embryos responsible for a heart condition. But their results were challenged soon after, wit
No country resorts to IVF more than Japan—or has less success
THE sterile façade of Kato Ladies Clinic gives little hint of the fecundity inside. Nestling among a plantation of high-rises in a business district of Tokyo, the clinic implants fertilised eggs in an average of 75 women a day. That makes it one of the busiest fertility hospitals in the world, says Keiichi Kato, the medical director.
The lab has been using pH monitoring with their equipment regularly and has determined that their pH values are within an acceptable range, even during frequent door openings. Based on this data, the lab asks, if the incubators are holding steady then why bother continuing with pH monitoring?
At SAFE Sens, we understand that pH inside an incubator can be a changing quantity. We have included here some pH profiles which have been observed by ourselves and others in order to help you diagnose what your pHs might mean.
In-line filter for cylinder and house gasses
O2, N2, CO2, and tri-gas. Aire~LifeLine provides in-line air filtration with top of the line protection, delivering a better and more consistent yield of cylinder and house gasses for a wide range of med
Vit Kit - Warm NX is an adaptable, cost-effective system for use in the thawing of oocytes, pronuclear zygotes, cleavage stage embryos, and blastocyst stage embryos. Unlike many vitrification kits that feature a mono-buffered system and M199 base med
Vit Kit - Freeze NX is an adaptable, cost-effective system for use in the vitrification of oocytes, pronuclear zygotes, cleavage stage embryos, and blastocyst stage embryos. Vit Kit - Freeze NX is the latest advancement in vitrification media aimed t
S-Cryolock is the slimmer version of the original. It is a versatile, simple and efficient vitrification device that is intended for the holding, cryopreservation and storage of oocytes or embryos in liquid nitrogen.
Source
Irvine Scientific
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